Application of flow cytometry in the differential diagnosis of lymphoma/leukemia with aberrant antigen expression.
- Author:
Cheng-Qing XIA
1
;
Shi-Lun CHEN
;
Man QI
;
Xian-Fa XU
Author Information
- Publication Type:Journal Article
- MeSH: Antigens, CD; metabolism; Antigens, Differentiation, Myelomonocytic; metabolism; CD13 Antigens; metabolism; CD3 Complex; metabolism; CD5 Antigens; metabolism; CD79 Antigens; metabolism; Diagnosis, Differential; Flow Cytometry; Humans; Leukemia, B-Cell; diagnosis; immunology; Leukemia, T-Cell; diagnosis; immunology; Lymphoma, Mantle-Cell; diagnosis; immunology; Peroxidase; metabolism; Retrospective Studies; Sialic Acid Binding Ig-like Lectin 3
- From: Chinese Journal of Pathology 2004;33(6):532-535
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the application of flow cytometry in the differential diagnosis of lymphoma/leukemia with aberrant antigen expression.
METHODSThe results of flow cytometry of 30 lymphoma/leukemia cases with aberrant antigen expression, of which 3 cases being lymphomas, 8 B-cell leukemia, 1 T-cell leukemia, 17 acute non-lymphoid leukemia and 1 acute non-lymphoid leukemia involving lymph nodes were analyzed. Immunohistochemistry (EnVision) for CD79a, CD3 and MPO was performed on all cases.
RESULTSEleven cases of B-cell lymphoma/leukemia were cytoplasmic CD79a (cCD79a)-positive, cytoplasmic CD3 (cCD3epsilon) and cytoplasmic MPO (cMPO)-negative. Five of these cases were positive for CD5 and 2 for CD5, 1 or 2 for myeloid marker(s). The T-cell leukemia cases were cCD3epsilon-positive, cCD79a and cMPO-negative, they also co-expressed CD13 and CD33. The mantle cell lymphoma cases were positive for CD3, CD13 and CD33. Of the 8 B-cell leukemia cases, 4 were positive for CD5, 3 for CD13 and 1 for CD13 and CD33. The 18 acute non-lymphoid leukemia cases (including 1 acute non-lymphoid leukemia case involving lymph nodes) were cMPO-positive and cCD79a and cCD3epsilon-negative. Eight of the 18 expressed T-cell markers (including 1 case of acute non-lymphoid leukemia involving lymph nodes), 8 expressed B-cell markers, 2 expressed both T and B-cell markers.
CONCLUSIONSFlow cytometry can demonstrate aberrant antigen expression in lymphoma/leukemia cells and is helpful in delineating their cell origin. The technique is thus useful in the differential diagnosis of lymphoma/leukemia.
