Effect of combination glycyrrhiizin and triptolide on TNF-alpha and IL-10 in serum of collagen induced arthritis rats.
- Author:
Wan-Dong ZHANG
1
;
Cheng LU
;
Hong-Yan ZHAO
;
Shi-Lin CHEN
;
Da-Jian YANG
;
Ai-Ping LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Inflammatory Agents; administration & dosage; isolation & purification; pharmacology; Anti-Inflammatory Agents, Non-Steroidal; administration & dosage; isolation & purification; pharmacology; Arthritis, Experimental; blood; chemically induced; pathology; Collagen Type II; Diterpenes; administration & dosage; isolation & purification; pharmacology; Drug Combinations; Epoxy Compounds; administration & dosage; isolation & purification; pharmacology; Glycyrrhizic Acid; administration & dosage; isolation & purification; pharmacology; Immunoglobulin G; blood; Interleukin-10; blood; Male; Phenanthrenes; administration & dosage; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; Tripterygium; chemistry; Tumor Necrosis Factor-alpha; blood
- From: China Journal of Chinese Materia Medica 2007;32(5):414-417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of combination therapy with glycyrrhizin (GL) and triptolide (TP) on collagen-induced arthritis (CIA) rats.
METHODSixty male SD rats were randomly divided into 6 groups: the model group, the TP group, the GL group, and combination 1, 2, 3 groups. The models were induced by collagen type II. The arthritis index (AI) and the edema rate were detected as curative effect, and the level of antibodies to collagen, TNF-alpha and IL-10 were measured by ELISA.
RESULTThe combination therapy with GL and TP significantly reduced the paw edema and arthritis index of CIA rats (P <0. 01 ), and the combination therapy can increase the level of IL-10, while decrease the level of TNF-alpha, and the level of antibodies to collagen decreased too (P <0.05, P <0.01).
CONCLUSIONCombine 26.78 mg x kg(-1) GL with 13.40 microg x kg(-1) TP can significantly inhibited the CIA, and the effect equal to the dosage of 17. 86 microg x kg(-1) TP. It supports the possible of GL in combination with TP to reduce the dose and side effects related to TP.
