Determination of calceolarioside B in Caulis Akebiae by RP-HPLC.
- Author:
Hui-Min GAO
1
;
Zhi-Min WANG
;
Li QU
;
Xue-Tao FU
;
Lin LI
Author Information
- Publication Type:Journal Article
- MeSH: Caffeic Acids; analysis; chemistry; China; Chromatography, High Pressure Liquid; methods; Ecosystem; Glucosides; analysis; chemistry; Magnoliopsida; chemistry; classification; growth & development; Plant Leaves; chemistry; Plant Roots; chemistry; Plant Stems; chemistry; Plants, Medicinal; chemistry; classification; growth & development; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(6):476-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a quantitative method for calceolarioside B in Caulis Akebiae.
METHODThe samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm.
RESULTThe calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%.
CONCLUSIONThe method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
