In vitro studies on asexual embryos and regenerated plantlets obtained from leaf organ of Panax notoginseng.
- Author:
Hong-Yuan XU
1
;
Ai-Dong MENG
;
Bing HE
;
Feng-Jue ZHOU
;
Tao-Ju LAN
;
Bo SHA
;
Hong-Zhang XU
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; pharmacology; Panax notoginseng; embryology; physiology; Plant Growth Regulators; pharmacology; Plant Leaves; embryology; physiology; Plants, Medicinal; embryology; physiology; Regeneration; drug effects; physiology; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2007;32(6):481-483
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study and improve the tissue culture technology of Panax notoginseng.
METHODUsing the callus of leaf blade and leafstalk of P. notogingseng as explants, MS + 2, 4-D 1.5 mg x L(-1) as basal medium, the formation of asexual embryos was induced by added LFS, BA, KT or ZT 0.5 mg x L(-1), and cultured in dark. It cultured then in 2000 lx of illumination for 10-12 h x d(-1) to induce the asexual embryos germinating and developing to be the regenerated-plantlet.
RESULT AND CONCLUSIONOnly the medium added with LFS could induce the formation of asexual embryos, and made it developed to be regenerated-plantlet. The inducing ratio of asexual embryos reached about 85%, and 30% of asexual embryos could grow and develop as robust regenerated-plantlets.
