Determination of aisaticoside in Centella asiatica extract by HPLC-ELSD.
- Author:
Quan-Lin YU
1
;
Wen-Yuan GAO
;
Hai-Xia CHEN
;
Hong-Quan DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Centella; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; chemistry; standards; Light; Molecular Structure; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results; Scattering, Radiation; Triterpenes; analysis; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(6):503-505
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA HPLC-ELSD method was established for the simultaneous quantitative determination of asiaticoside in Centella asiatica extract.
METHODThe column was packed with 5 m HIQ C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L min(-1).
RESULTThe linear ranges of the asiaticoside were 0.35-7.0 microg. The recovery of the asiaticoside in C. asiatica extract was 94.9% (RSD 1.7%).
CONCLUSIONThe method is reliable, simple, precise and could be used for the quality control of C. asiatica extract.
