Determination of butylidenephthalide in Ligusticum chuanxiong by HPLC.
- Author:
Cheng-Yuan WANG
1
;
Hao ZHANG
;
Zhong-Ming QIAN
Author Information
- Publication Type:Journal Article
- MeSH: China; Chromatography, High Pressure Liquid; instrumentation; methods; Light; Ligusticum; chemistry; Phthalic Anhydrides; analysis; Plants, Medicinal; chemistry; Reproducibility of Results; Rhizome; chemistry; Scattering, Radiation
- From: China Journal of Chinese Materia Medica 2007;32(6):508-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine butylidenephthalide in Ligusticum Chuanxiong with RP-HPLC.
METHODThe sample was extracted with methanol using sonication. The ESTD was used to quantify butylidenephthalide. HPLC separation was carried out in a Hypersil ODS columm (4.6 mm x 150 mm, 5 microm) , eluted at 1 mL x min(-1) with methanol-5% isopropyl alcohol (60: 40) at 25 degrees C. The detection wavelength was 230 nm.
RESULTThe linear range was 0.07-0.7 microg for butylidenephthalide. The average recovery was 95.3%, and RSD was 2.3% (n =6).
CONCLUSIONThis method was simple and could be used to determine butylidenephthalide with satisfactory accuracy and reproducibility.
