Comparison of two preparation methods applied in tanshinone II(A)-loaded PLGA nanoparticles.
- Author:
Liang-Chun GAN
1
;
Shi-Xiang HOU
;
Yue-Qi BI
;
Chang-Guang WANG
;
Xin-Chun WANG
;
Qi-Xiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Chemical Precipitation; Crystallization; Diterpenes, Abietane; Emulsions; Lactic Acid; chemistry; Nanoparticles; chemistry; Particle Size; Phenanthrenes; chemistry; isolation & purification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Polyglycolic Acid; chemistry; Polymers; chemistry; Quality Control; Salvia miltiorrhiza; chemistry; Technology, Pharmaceutical; methods; Volatilization
- From: China Journal of Chinese Materia Medica 2007;32(7):578-581
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize formulation of tanshinone II(A)-loaded PLGA nanoparticles and compare the difference of two methods in preparation and quality of nanoparticles.
METHODThe two methods were nanoprecipitation method and emulsion-evaporation method. Single factor experiments and central composite design and response surface method were used to optimize the formulation of nanoparticles. The nanoparticles were characterized at size, morphology, entrapment efficiency, drug loading, drug recovery rate, crystallinity and drug release in vitro.
RESULTThe mean diameters were 225 nm and 183 nm, the entrapment efficiency were 95.49% and 87.99%, the drug loading were 2.03% and 0.16%, and the drug recovery rates were 38.42% and 17.59% respectively for nanoprecipitation method and emulsion-evaporation method.
CONCLUSIONNanoprecipitation method was better than emulsion-evaporation method for preparation of tanshinone II(A)-loaded PLGA nanoparticles.
