Technique of macroporous resin for tartary buckwheat total flavonoids.
- Author:
Zhi-Feng YU
1
;
Min WANG
Author Information
- Publication Type:Journal Article
- MeSH: Fagopyrum; chemistry; Flavonoids; isolation & purification; Plants, Medicinal; chemistry; Resins, Synthetic; chemistry; Seeds; chemistry; Technology, Pharmaceutical; methods
- From: China Journal of Chinese Materia Medica 2007;32(7):585-589
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigatre appropriate macroporous resins and the optimal technological parameters of the purification process of tartary buckwheat total flavonoids.
METHODStatic and dynamic adsorption-desorption methods were adopted, and evaluated for separating efficiency by macroporous resins absorption rates, desorption rates.
RESULTThe DM -2 macroporous resin had the best separating efficiency. The best absorbed condition is H2O as solvent of extracts, diameter vs height: 1: 10, pH 3 - 4, absorption power: 3.0 mL x min(-1). The optimum adsorption condition is the volume of 50% ethanol 80 mL (approximately 5 BV (resin bed volume) as eluting solvent, pH 8, the desorption power: 3.0 mL x min(-1).
CONCLUSIONThis technology is simple, quick and cost-effective, which is suitable for industrialization.
