Effect on expression of mice alpha-hemoglobin stabilizing protein in different developmental stages treated with Yisui Shengxue granules.
- Author:
Yong-Mei LIU
1
;
Zhi-Kui WU
;
Li-Min CHAI
;
Xin-Hua ZHANG
;
Min LI
;
Yu-Ying CHEN
;
Xin-Xia LV
;
Xiao-Yun ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Proteins; genetics; Bone Marrow Cells; cytology; drug effects; metabolism; Drug Combinations; Drugs, Chinese Herbal; isolation & purification; pharmacology; Erythrocytes; cytology; drug effects; metabolism; Female; Gene Expression Regulation, Developmental; drug effects; Liver; cytology; embryology; metabolism; Male; Mice; Molecular Chaperones; genetics; Plants, Medicinal; chemistry; Pregnancy; RNA, Messenger; biosynthesis; genetics; Random Allocation; Spleen; cytology; embryology; metabolism; Up-Regulation; drug effects; genetics
- From: China Journal of Chinese Materia Medica 2007;32(7):609-612
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the effect of Yisui Shengxue granules on expression of alpha-hemoglobin stabilizing protein (AHSP) mRNA in different developmental stages mice.
METHODThe total RNAs were extracted from the bone marrow karyocyte of normal adult mice and the karyocyte of fetus liver and fetus spleen in pregnanted mice (pregnanted 21 days) and fetal mice (pregnanted 14 days). The expression level of AHSP mRNA in different developmental stages mice interfered with Yisui Shengxue granules was measured by real-time PCR.
RESULTThe intervention of Yisui Shengxue granules could significantly up-regulated the expression levels of AHSP mRNA in normal adult mice.
CONCLUSIONThe result revealed that one of possible molecular mechanism of the effects caused by Yisui Shengxue granules is that it can promote the AHSP gene expression, reduce the free a-globin deposit, then prevent the poison to erythrocyte and decrease the haemolysis.
