Effect of Erythrina variegata on Ca2+ homeostasis in ovariectomized rats and action mechanism.
- Author:
Yan ZHANG
1
;
Xiao-Li LI
;
Wen-Xiu HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Calcium; blood; metabolism; urine; Drugs, Chinese Herbal; isolation & purification; pharmacology; Duodenum; drug effects; metabolism; Erythrina; chemistry; Female; Gene Expression; drug effects; Homeostasis; drug effects; Kidney; drug effects; metabolism; Ovariectomy; Plant Bark; chemistry; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Receptors, Calcitriol; genetics; Reverse Transcriptase Polymerase Chain Reaction; S100 Calcium Binding Protein G; genetics
- From: China Journal of Chinese Materia Medica 2007;32(7):627-630
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of Erythrina variegata (EV) on Ca2+ homeostasis in ovariectomized rats and the regulation on gene expression in duodenum and kidney.
METHODFour weeks after surgical operation, the ovariectomized (OVX) rats were administered orally with estradiol and EV extracts for 14 weeks. Ca level in serum and urine was measured by colorimetric methods, and gene expressions in duodenum and kidney were analyzed by reverse transcriptase-polymerase chain reaction (RT-PCR).
RESULTEV extracts could improve the serum Ca level and inhibite the urinary Ca excretion in OVX rats, and this might be due to the upregulation of EV on VDR mRNA expression in duodenum and CaBP-9k mRNA expression in kidney.
CONCLUSIONEV could maintain Ca homeostasis in OVX rats.
