PCR, clone and sequence analysis of rDNA-ITS of Nelumbo nucifera from different geographical origins in China.
- Author:
Shan LIN
1
;
Wei-wen ZHENG
;
Jin-zhong WU
;
Li-juan ZHOU
;
Ya-na SONG
Author Information
- Publication Type:Journal Article
- MeSH: Base Sequence; China; DNA, Plant; chemistry; genetics; metabolism; DNA, Ribosomal Spacer; classification; genetics; Deoxyribonuclease EcoRI; metabolism; Deoxyribonucleases, Type II Site-Specific; metabolism; Drug Contamination; prevention & control; Geography; Nelumbo; classification; genetics; Phylogeny; Plants, Medicinal; classification; genetics; Polymerase Chain Reaction; RNA, Ribosomal; genetics; RNA, Ribosomal, 18S; genetics; RNA, Ribosomal, 5.8S; genetics; Sequence Analysis, DNA; Species Specificity
- From: China Journal of Chinese Materia Medica 2007;32(8):671-675
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo provide DNA molecular marker for identification of Nelumbo nucifera by exploring the differences of nrDNA-ITS sequence of N. nucifera originated from different habitats.
METHODTo compare nrDNA-ITS base sequence using specific PCR-ITS.
RESULTThe completed sequence of ITS and 5.8 S rDNA, and the partial sequences of 18S rDNA and 26S rDNA, totally 750 bp, from N. nucifera were obtained. The differences among N. nucifera from different habitats and from different cultivars were found.
CONCLUSIONThe method can be used to identify N. nucifera among different species and to distinguish their fakes. It provided the basis for identifying N. nucifera from different geographical regions by comparison of their ITS sequences.
