Study on residue analysis method of imidacloprid in Ephedrae sinica.
- Author:
Mei-yan CHEN
1
;
Jun CHEN
;
Jian XUE
;
Jing YU
;
Hui-zhen CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Ephedra sinica; chemistry; Imidazoles; analysis; Insecticides; analysis; Neonicotinoids; Nitro Compounds; analysis; Pesticide Residues; analysis; Plants, Medicinal; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2007;32(8):675-677
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish residue analysis method of imidacloprid in Ephedra sinica by HPLC.
METHODImidacloprid was extracted with dichloromethane, cleaned up with chromatography column, then separated on Spherisorb C18 column by using Methanol-water (20:80), detected at 270 nm.
RESULT AND CONCLUSIONThe limit of detection (LOD) and limit quantification (LOQ) were 0.4 x 10(-9) g and 0.02 mg x kg(-1), mean recovery and related standard deviation (RSD) were 85.37%-90.65% and 2.23%-3.45%. It is concluded that the method could satisfy the pesticide residue analysis demands in sensitivity, accuracy and precision.
