Detection of monoamine transmitters in serum of macaque by high performance liquid chromatograghy with electrochemical detector.
- Author:
Fang LI
1
;
Hui-yun ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biogenic Monoamines; blood; Chromatography, High Pressure Liquid; methods; Dopamine; blood; Electrochemistry; methods; Epinephrine; blood; Female; Humans; Macaca; Norepinephrine; blood; Premenstrual Syndrome; blood; Reproducibility of Results; Serotonin; blood
- From: China Journal of Chinese Materia Medica 2007;32(8):703-705
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC-ECD for the determination of monoamine transmitters in serum of macaque.
METHODThe analysis was carried out on a ZORBAX SB-C18 column (4.6 mm x 250 mm, 5 microm) eluted with a mobile phase of methanol-water (18:82) at a flow rate of 0.9 mL x min(-1).
RESULTThe recoveries of NE, E, DA, 5-HT were 97.0%, 97.8%, 99.5%, 100.3%, RSD was 0.22%-0.93%, and the repeatability was good.
CONCLUSIONThe results prove that the method is simple, fast, accurate and can be used to determine simultaneously the concentration of monoamine transmitters in serum of macaque.
