Study on chromatographic fingerprint of extractive from Tripterygium wilfordii with supercritical carbon dioxide.
- Author:
Wen ZHOU
1
;
Hong-ru LI
;
Shu-fen LI
;
Yoshihisa TAKAISHI
;
Hong-quan DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Carbon Dioxide; chemistry; Chromatography, High Pressure Liquid; methods; Chromatography, Supercritical Fluid; methods; Plant Extracts; analysis; chemistry; isolation & purification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tripterygium; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(8):706-709
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the chromatographic fingerprint of supercritical carbon dioxide extract of Tripterygium wilfordii.
METHODHPLC method was applied for quality assessment of T. Wilfordii, HPLC analysis was performed on Kromasil C18 (4. 6 mm x 250 mm, 5 microm) with the mixture of acetonitrile-1% per thousand H3PO4, as mobile phase in gradient mode. The samples were detected at UV of 267 nm with column temperature of 35 degrees C, analytic time was 80 min; Flow-rate was 1.0 mL x min(-1). The chromatographic fingerprint of ten batches of samples was determined, for establishing the chromatographic fingerprint of T. Wilfordii.
RESULTIndicating 27 peaks in common, identified 21 peaks with chemical reference and HPLC-MS, and the HPLC fingerprint was established.
CONCLUSIONThe method is steady and accurate with a good repeatability and can be used as a quality control method for T. Wilfordii.
