Comparison of antioxidative and antitumor activities of six flavonoids from Epimedium koreanum.
- Author:
Ting WANG
1
;
Jin-chao ZHANG
;
Yao CHEN
;
Feng HUANG
;
Meng-su YANG
;
Pei-gen XIAO
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; isolation & purification; pharmacology; Antioxidants; isolation & purification; pharmacology; Breast Neoplasms; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Dose-Response Relationship, Drug; Epimedium; chemistry; Female; Flavonoids; isolation & purification; pharmacology; Humans; Liver Neoplasms; pathology; Luteolin; isolation & purification; pharmacology; Plants, Medicinal; chemistry
- From: China Journal of Chinese Materia Medica 2007;32(8):715-718
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the antioxidative and antitumor activities of flavonoids isolated from Epimedium koreanum.
METHODThe compounds were separated by column chromatography with silica gel and Sephadex LH-20, and identified by spectral a- nalysis (ESI-MS, 1H-NMR and 13C-NMR) respectively. DPPH radical scavenging assay and MTT assay were used to observe the antioxidative and antitumor abilities.
RESULTSix compounds were isolated from the the ethyl acetate extract of the aerial part. Their structures were identified as icariin (I), luteolin (II), baohuoside II (III), hyperoside (IV), epimedokoreanin B (V) and baohuoside I (VI). The results indicated that at concentrations of 3. 125-200 micromol x L(-1), compound I, III and VI had no ability to scavenge the DPPH radical, but the scavenging ability of compounds II, IV and V were stronger than that of Vit C in dose-dependant manner. Compounds I, II, V and VI could inhibit the proliferation of MCF-7 and HepG2 in dose-dependant manner, but compounds III and IV had no effect on the proliferation.
CONCLUSIONThe antitumor activity of E. koreanum may be partially related to the antioxidantive activity of flavonoids.
