Proteomic analysis of differentially expressed proteins in human hepatoma SMMC-7721 cells induced by Fufang Banmao capsule serum.
- Author:
Yong-Yan CAO
1
;
Meng WANG
;
Kai-Shun HUANG
;
Qiang YANG
;
Min DING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Annexin A5; metabolism; Antineoplastic Agents; isolation & purification; pharmacology; Carcinoma, Hepatocellular; metabolism; pathology; Cell Line, Tumor; Coleoptera; chemistry; Drug Combinations; Electrophoresis, Gel, Two-Dimensional; Female; HSP70 Heat-Shock Proteins; metabolism; Humans; Liver Neoplasms; metabolism; pathology; Male; Materia Medica; isolation & purification; pharmacology; Peptide Initiation Factors; metabolism; Peroxiredoxins; metabolism; Proteome; drug effects; metabolism; Proteomics; methods; RNA-Binding Proteins; metabolism; Rabbits; Serum; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: China Journal of Chinese Materia Medica 2007;32(9):831-834
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Fufang Banmao capusle on the proteome of SMMC-7721 cells and discover potential molecular mechanism of anti-cancer at molecular level.
METHODSMMC-7721 cells were treated by Fufang Banmao capusle serum prepared with serum pharmacological method; proteomic protocol involving 2-DE, image analysis and mass spectrometry were used to detect the proteins in cells influenced by Fufang Banmao capusle.
RESULTApproximately 450 protein spots in SMMC-7721 cells were resolved and detected in 2-D gel maps from pH3-10L IEF. 47 protein plots varied over 2-fold quantitively between treated sample and control sample were uncovered. 13 differentially expressed proteins spots were further identified by MALDI-TOF-MS analysis and four of them were successfully identified. Annexin A5, heatshock 70 x 10(3) protein 8 was significantly up-regulated in treated sample compared with control sample, while Eukaryotic translation initiation factor 5A and Peroxiredoxin-2 was significantly down-regulated in treated sample.
CONCLUSION4 differently expressed proteins associated with the proliferation, apoptosis, immunity of tumor were detected and they might provide clues for the coming research. The protocol of proteomics combined with serum pharmacological method is an effective platform to research complicated formulas in that it is capable of laying out many proteins associated with Fufang Banmao capusle.
