A Preliminary Study on the Expression of C-X-C Chemokine Genes in Colonic Mucosa of Patients with Ulcerative Colitis.
- Author:
Suk Kyun TANG
;
Ok Hee KIM
;
Me Hwa LEE
;
Ki Nam SHIM
;
Eui Ryun PARK
;
Hwoon Yong JUNG
;
Weon Seon HONG
;
Young Il MIN
- Publication Type:Original Article
- Keywords:
Chemokine;
Ulcerative colitis;
Colon
- MeSH:
Biopsy;
Chemokines;
Chemokines, CXC;
Colitis, Ulcerative*;
Colon*;
Humans;
Inflammation;
Mucous Membrane*;
RNA;
RNA, Messenger;
Ulcer*
- From:Journal of the Korean Society of Coloproctology
1998;14(4):735-742
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Chemokines are potent regulators of the host inflammatory or immune responses. Mucosal synthesis of chemokines may be important in the pathogenesis of mucosal inflammation in ulcerative colitis (UC). We performed this study to investigate the expression of C-X-C chemokine genes in UC. METHODS: Mucosal tissues were obtained from six normal controls and six UC patients by endoscopic biopsies. In patients with UC, mucosal tissues were separately obtained from both involved and uninvolved regions. RNA was extracted and mRNA levels of five C-X-C chemokines were determined by quantitative reverse transcription-PCR using internal RNA standards. RESULTS: Mucosal mRNA levels of all chemokines tested increased in the involved region of UC compared with the uninvolved region of UC or normal controls. CONCLUSION: Our data suggest that mucosal expression of C-X-C chemokines contributes to the pathogenesis of UC
