Inhibition of serine/threonine kinase 15 gene expression induces apoptosis of gastric cancer cells.
- Author:
Bin LAN
1
;
Bing-ya LIU
;
Xue-hua CHEN
;
Ying QU
;
Xiao-qing ZHANG
;
Qu CAI
;
Qi-bao DAI
;
Jian ZHANG
;
Zheng-Gang ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Aurora Kinase A; Aurora Kinases; Cell Line, Tumor; Gene Expression Regulation, Neoplastic; Gene Silencing; Humans; Protein-Serine-Threonine Kinases; genetics; RNA, Small Interfering; Stomach Neoplasms
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(5):417-420
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of inhibition of serine/threonine kinase15 (STK15) gene expression on apoptosis induction in gastric cancer cell line-MKN45 and discuss the role of STK15 in viability of gastric cancer cells.
METHODSThe STK15 expression was inhibited by chemically synthesized siRNA. The STK15 mRNA and protein level were respectively measured by real-time quantitative PCR and western blotting,the change of cell cycle distribution and apoptosis rate were detected by flow-cytometry, cell morphological change was observed by Hoechst staining,and pro-caspase 3 level was also detected by western blot.
RESULTSAfter treatment by siRNA targeting STK15 after 48 h, STK15 mRNA and protein level decreased obviously. More MKN45 cells accumulated at G(2)/M phase (P< 0.05). The apoptosis rate of STK15 siRNA treated MKN45 cells was higher than that of control cells(P< 0.05) with the pro-caspase 3 level decreased.
CONCLUSIONSInhibition of STK15 gene expression may induce apoptosis in MKN45 cells through the pathway of caspase3. STK15 gene play a key role in proliferation and viability of MKN45 cells.
