Expression of mucosal addressin cell adhesion molecule-1 during small bowel graft rejection in rat.
- Author:
Ji LIU
1
;
Wei-zhong WANG
;
Wen-xian GUAN
;
Dong-li CHEN
;
Ji-peng LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Graft Rejection; immunology; metabolism; Graft Survival; Immunoglobulins; metabolism; Intestinal Mucosa; immunology; Intestine, Small; transplantation; Lymph Nodes; metabolism; Lymphoid Tissue; Male; Mucoproteins; metabolism; Rats; Rats, Inbred BN; Rats, Inbred F344
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(5):421-424
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of mucosal addressin cell adhesion molecule-1(MAdCAM-1) during small bowel graft rejection and the effects of MAdCAM-1 on the development of acute rejection.
METHODSRat heterotopic small bowel transplantation (SBT) was performed in F344/N rats with syngeneic and allogeneic (BN-F344/N) grafts. Bowel and gut-associated lymphoid tissue(GALT) samples were collected from small bowel transplants on postoperative day(POD) 1, 3, 5 and 7. Histopathology assessment of the grafts was conducted to identify the rejection. MAdCAM-1 was detected by immunohistochemistry and Western blot.
RESULTSDuring acute rejection, MAdCAM-1 was highly-expressed on gut lamina propia and GALTs, particularly on vascular endothelial cells in the gut lamina propia. There were no change of MAdCAM-1 expression in syngeneic grafts from POD1 to POD7. In allogeneic grafts, MAdCAM-1 expression in mesenteric lymph nodes was down-regulated, while up-regulated on the vascular endothelial cells in the lamina propria during acute rejection.
CONCLUSIONAlteration of MAdCAM-1 expression may be associated with the development of SBT graft rejection.
