Expression of Ezrin in gastric carcinoma and its significance.
- Author:
Rong-liang SHI
1
;
Jian-fang LI
;
Ying QU
;
Xue-hua CHEN
;
Qin-long GU
;
Zheng-gang ZHU
;
Bing-ya LIU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Cytoskeletal Proteins; genetics; metabolism; Female; Gene Expression; Humans; Lymphatic Metastasis; Male; Neoplasm Invasiveness; Neoplasm Staging; Protein Array Analysis; Stomach Neoplasms; metabolism; pathology
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(5):433-435
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of Ezrin in gastric cancer, its role in tumor metastasis.
METHODSEzrin expression in tumor tissues from 90 gastric cancer cases and in normal gastric mucosa from 12 cases with benign disease was examined by immunohistochemical staining. Ezrin expression in gastric cancer cell lines was also detected by Western blot, and in vitro invasion assay was used to examine the invasive ability of the cell lines.
RESULTSThe expression rate of Ezrin was significantly higher in gastric cancer tissues than that in normal tissues (P< 0.05), and significantly correlated with lymph node metastasis (P< 0.05). Western blot showed that MKN-45 cell line had the highest expression of Ezrin among 5 gastric cancer cells. MKN-45 possessed highest invasion ability.
CONCLUSIONEzrin expression is up-regulated, and may be associated with lymph node metastasis in gastric cancer.
