Effect of 5-fluorouracil in combination with Astragalus membranaceus on amino acid metabolism in mice model of gastric carcinoma.
- Author:
Zhi-xiang ZHANG
1
;
Feng QI
;
De-jun ZHOU
;
Xiao-yu LIANG
;
Li-wei ZHU
;
Peng-zhi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acids; metabolism; Animals; Astragalus membranaceus; Fluorouracil; therapeutic use; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Phytotherapy; Stomach Neoplasms; drug therapy; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(5):445-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of 5-fluorouracil-FU in combination with astragalus membranaceus(AM) on amino acid metabolism in mice model of gastric carcinoma induced by 3-methylcholanthrene(MC).
METHODSMice gastric carcinoma models were established by 3-methylcholanthrene induction and randomly divided into different groups, and received 5-FU treatment (group A) 5-FU plus AM (group B), 5-FU plus a high dose of AM(group C), no treatment (group D). Normal mice were used as control (group N). Free amino acid in the tumor specimens were examined.
RESULTSThe levels of free Valine, Methionine, Leucine, Arginine and cystine in the tumor specimens in group D were significantly higher than that in group N(P< 0.05). The levels of free serine in group A, B, C, D were significantly higher than that in group N. The levels of free glutamic acid in group A, B were significantly higher than that in group N(P< 0.05). The levels of free proline in group C, D were significantly higher than that in group P, N(P< 0.05).
CONCLUSIONSThe increasing levels of free serine and proline in tumor specimens in gastric cancer mice model reveals metabolic disturbance of amino acid. 5-FU plus astragalus membranaceus can decrease the level of free glutamic acid in the mice models, and inhibit tumor growth.
