Study on the effects of mitochondrial pathways on apoptosis in colon carcinoma cells induced by tumor necrosis factor related apoptosis inducing ligand.
- Author:
Ji-hui HAO
1
;
Ming YU
;
Qiang LI
;
Yu-rong SHI
;
Yi YANG
;
Xi-shan HAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cardiolipins; metabolism; Caspase 9; metabolism; Cell Line, Tumor; Cytochromes c; metabolism; Humans; Mitochondria; metabolism; Mitochondrial Membranes; metabolism; Receptors, TNF-Related Apoptosis-Inducing Ligand; metabolism; TNF-Related Apoptosis-Inducing Ligand; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(6):519-522
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effects of mitochondrial pathways on apoptosis in colon carcinoma cells induced by Tumor necrosis factor related apoptosis inducing ligand and offer evidences for TRAIL application in clinic.
METHODSApoptosis, integration of mitochondria (including DeltaPsim, cardiolipin), activity of Caspase-9 and release of cytochrome c in colon carcinoma cells SW1116 treated with TRAIL, were detected by means of flowcytometry, fluorometer method and western-blot at the different time point.
RESULTSAfter treated with TRAIL for 4 hours, the apoptosis index was 32.98%, and the damage of mitochondria occurred with DeltaPsim, cardiolipin decreased, and the activity of Caspase-9 and cytochrome c increased. The Caspase-9 activity at 24 hour was (48.12+/-2.21)micromol.L(-1).h(-1).mg(-1) protein. Mitochondrial damage induced by TRAIL could be inhibited by Caspase inhibitor Z-VAD. fmk.
CONCLUSIONMitochondrial pathways involved in the apoptosis of colon carcinoma cell induced by TRAIL. Cytochrome c was released and Caspase-9 was activated in the Caspase-dependent manner after the damage of mitochondrial.
