Effects of enteral nutrition on intestinal intraepithelial lymphocytes of intestinal mucosal and the barrier of mucus in patients with stomal type enteric fistula.
- Author:
Shu-jian HONG
1
;
Guo-sheng GU
;
Jian-an REN
;
Ning LI
;
Jie-shou LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Cell Count; Enteral Nutrition; Female; Humans; Intestinal Fistula; physiopathology; therapy; Intestinal Mucosa; immunology; physiopathology; Intestine, Small; Lymphocytes; metabolism; Male; Middle Aged; Mucus; immunology; Young Adult
- From: Chinese Journal of Gastrointestinal Surgery 2006;9(6):527-529
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of enteral nutrition on intestinal intraepithelial lymphocytes and the barrier of mucus in patients with stomal type enteric fistulas.
METHODSTen patients with stomal type enteric fistulas after long-term fasting were observed. They received enteral nutrition of 146 kJ.kg(-1).d(-1) non-protein calorie and 0.25 g.kg(-1).d(-1) nitrogen per day. Intestinal mucosa were taken by endoscope through stoma of fistula before, 5 and 10 days after enteral nutrition support. Hematoxylin-eosin stain and immunohistochemical stain were performed to count the cell counts of intestinal intraepithelial lymphocytes (iIELs) and mucin-2 (MUC2) positive cells, specific stain (Alcian Blue) was performed to test the thickness of mucus.
RESULTSFive days after enteral nutrition, MUC2 positive cells and the thickness of mucus were significantly higher than that before enteral nutrition support (P<0.05). Ten days after enteral nutrition, iIEL cell and CD8 counts were also significantly higher than that before enteral nutrition support (P<0.05), MUC2 positive cells and the thickness of mucus showed a significant increase (P<0.01).
CONCLUSIONEnteral nutrition is effective in protecting the gut mucosal and improving the immune function of the intestinal intraepithelial in patients with stomal type fistula.
