OBJECTIVETo establish the techniques for the purification of Huanglian Jiedu decoction by macroporous resins combinations, and to lay the foundation of improvement of dosage forms in clinical application and realization of industrialized production about Huanglian Jiedu decoction.

METHODFour kinds of macroporous resins (X-5, S-8, HPD100, AB-8) were chosen and combined in six different forms. Adsorption and desorption experiments were carried out and contents of six index constituents, gardenoside, baicalin, palmatine, berberine, baicalein and wogonin, in different groups were compared before and after purification.

RESULTAdsorption rates of six index constituents in Huanglian Jiedu decoction of different combinations of macroporous resins groups were all nearly 100%, but desorption rates had remarkable differences.

CONCLUSIONThrough comprehensive comparison, macroporous resins combination 6 resulted in high contents of index suitable relatively. It is suitable for purification of Huanglian Jiedu decoction.