Determination of underivatized glycine and proline in vinegar turtle shell by HPLC-ELSD.
- Author:
Xinyu WANG
1
;
Xiaomei TAN
;
Mingze GAO
;
Jinlong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Animal Shells; chemistry; Animals; Chromatography, High Pressure Liquid; Glycine; analysis; Proline; analysis; Quality Control; Reference Standards; Reproducibility of Results; Turtles
- From: China Journal of Chinese Materia Medica 2011;36(15):2107-2109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a method to determine the underivatized glycine (Gly) and proline (Pro) in vinegar turtle shell.
METHODAn HPLC-ELSD method was conducted on a Prevail C18 column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile and 0.7% trifluoroacetic acid solution (containing 5.0 mmol x L(-1) heptafluorobutyric acid), and elution time was 15 min.
RESULTThe calibration curves were showed good linearity within the concentration range of 0.14-0.6 g x L(-1). The average recoveries were 101.2% and 102.5%, and RSD were 1.9% and 2.5%, respectively.
CONCLUSIONSince this method needs neither the special amino acid analyzer nor derivation of amino acid., it is efficient, simple and accurate., which could be used for quality control of vinegar turtle shell.
