Effects of isopsoralen on bone marrow stromal stem cells differentiate and proliferate in vitro.
- Author:
Leiguo MING
1
;
Baofeng GE
;
Keming CHEN
;
Huiping MA
;
Yuankun ZHAI
Author Information
- Publication Type:Journal Article
- MeSH: Alkaline Phosphatase; metabolism; Animals; Bone Marrow Cells; cytology; drug effects; metabolism; Cell Differentiation; drug effects; genetics; Cell Proliferation; drug effects; Core Binding Factor Alpha 1 Subunit; genetics; Fibroblast Growth Factors; genetics; Furocoumarins; pharmacology; Gene Expression Regulation; drug effects; Insulin-Like Growth Factor I; genetics; Male; Mesenchymal Stromal Cells; cytology; drug effects; metabolism; Osteocalcin; metabolism; Osteogenesis; drug effects; RNA, Messenger; genetics; Rats; Stromal Cells; cytology; drug effects; metabolism; Transcription Factors; genetics
- From: China Journal of Chinese Materia Medica 2011;36(15):2124-2128
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigated the effects of isopsoralen on bone marrow stromal stem cells (BMSCs) differentiate and proliferate in vitro.
METHODThe stratum of mononuclear cells were separated and collected from the rat bone marrow sample by the all bone marrow cell culture methods. The cells were cultured in DMEM contained 10% fetal bovine serum. The culture medium was changed after three days. Nine days later, cells were treatment by isopsoralen with the concentration 1 x 10(-5), 1 x 10(-4), 1 x10(-6), 1 x 10(-7) mol x L(-1). MTT method was used for the proliferated analyzing. Under the induced condition, the alkaline phosphatase (ALP) activity, calcium salt sediment yield and osteocalcin were measured at the 4, 8, 12, 16 d. At the fifteenth day, histochemistry dyeing for calcified tubercle and ALP was proceeded. Total RNA was isolated and the gene expression of bFGF, IGF-1, Osterix and Runx-2 were investigated by Real Time PCR.
RESULTThe BMSCs proliferation refrained by isopsoralen with dose dependent. But it significantly enhanced osteogenesis, which was represented by the promotion of the ALP activity, calcium salt sediment yield, osteocalcin, and calcified tubercle amount. Besides, it also enhanced the mRNA level of bFGF, IGF-1, Osterix and Runx-2.
CONCLUSIONThe isopsoralen with the concentration 1 x 10(-5) mol x L(-1) can promote BMSCs differentiation to osteoblasts. It demonstrated the isopsoralen can prevent antiosteoporotic, which is an active part of the traditional Chinese medicine psoralea corylifolia.
