Activity evaluation of components and preparation of effective components group of xiaoxuming decoction for anti-cerebral ischemic.
- Author:
Yuehua WANG
1
;
Hailin QIN
;
Xiaoli HE
;
Guanhua DU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain; blood supply; drug effects; metabolism; Cell Survival; drug effects; Drugs, Chinese Herbal; pharmacology; therapeutic use; Free Radical Scavengers; pharmacology; therapeutic use; Glutamic Acid; adverse effects; Hydrogen Peroxide; adverse effects; Ischemia; drug therapy; metabolism; Male; Mitochondria; drug effects; metabolism; PC12 Cells; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2011;36(15):2140-2144
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEOn basis of preliminary studies, to prepare the effective components group of Xiaoxuming decoction for anti-ischemic by combining chemical analysis with pharmacological activity screening.
METHODFree radical scavenging was assayed by DPPH method; the cell viability injured by hydrogen peroxide, L-glutamate, and hypoxia was determined by MTT assay; TBA method was used to determine mitochondrial lipid peroxidation.
RESULTComprehensive analysis of multi-target results, the comprehensive activities of 40% ethanol elution and the middle layer were the highest.
CONCLUSION40% ethanol elution and the middle layer were proportionally mixed as the effective components group of Xiaoxuming decoction.
