Effects of PEG stress on flavonoids accumulation and related gene expression in suspension of Scutellaria baicalensis.
- Author:
Zhaochun YANG
1
;
Yuan YUAN
;
Min CHEN
;
Lingfei SHUAI
;
Qian XIAO
;
Shufang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Flavonoids; metabolism; Gene Expression; Polyethylene Glycols; pharmacology; Scutellaria baicalensis; drug effects; metabolism; Suspensions
- From: China Journal of Chinese Materia Medica 2011;36(16):2157-2161
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of PEG stress on baicalin, baicalein accumulation induced by an increased concentration of PEG solution and the related genes' expression in suspension of Scutellaria baicalensis.
METHODThe content of baicalin, baicalein in suspension of S. baicalensis was determined by HPLC. The related genes' expression was analyzed by semi-quantitative PCR.
RESULTThe content of proline in suspension of S. baicalensis was promoted by PEG treatment. Ten percent PEG treatment promoted the expression of PAL and the content of baicalein in experimental material via a drought stress. 20% PEG solution treatment promoted the expression of UBGAT. At the same time, the increased activity of APX inhibited the progress of eliminating reactive oxygen by baicalein, which induced the transformation from baicalein to baicalin.
CONCLUSIONActive ingredient in suspension of S. baicalensis was promoted significantly via a stress of light concentration of PEG solution.
