Extraction, purification technology and antineoplastic effects of solamargine.
- Author:
Zhaohui TANG
1
;
Yan ZHANG
;
Na LI
;
Lianming XU
;
Binjiang ZHAO
;
Wei XIAO
;
Zhenzhong WANG
;
Yu'an BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antineoplastic Agents, Phytogenic; isolation & purification; Cell Line, Tumor; Female; Humans; Mice; Mice, Inbred ICR; Solanaceous Alkaloids; isolation & purification; pharmacology
- From: China Journal of Chinese Materia Medica 2011;36(16):2192-2195
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo extract and purify of solamargine from Solanum nigrum, and to research its antineoplastic effects.
METHODS. nigrum was extracted refluently with 80% alcohol, solamargine was purified with silica gel column chromatography and recrystallization, and then conducted its structure identification and purity checks. Screened the effect on human tumor cell groth inhibition in vitro by MTT assay, and researched on the features in mice with H22 liver cancer or Ehrlich ascites tumor of solamargine.
RESULTThe concent of solamargine reached 97.9%. Solamargine had significantly inhibition on 6 tumor cells in vitro, and it had significantly inhibition on mice with H22 liver cancer or ehrlich ascites tumor in the 2.4 mg x kg(-1) dose of i.v.
CONCLUSIONSolamargine have the antineoplastic effect.
