Simultaneous determination of 4 nortriterpenoids in Schisandra chinensis extract by HPLC.
- Author:
Xiaoping HAN
1
;
Dekun LI
;
Dazheng ZHOU
;
Ruichao LIN
;
Lifang LIU
;
Zhengliang YE
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Schisandra; chemistry; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2011;36(16):2226-2229
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine 4 nortriterpenoids (de-hydroxy arisanlactone D, 25-hydroxy schindilactone, schindilachone A, lancifodilactone D) in Schisandra chinensis extract by HPLC.
METHODThe analysis was performed on a waters symmetry column (4.6 mm x 250 mm, 5 microm) with the mobile phase of acetonitrile-water (33:67) at a flow rate of 1 ml x min(-1). The column temperature was set at 37 degrees C, and the detector wavelength was 264 nm.
RESULTThe linear ranges of de-hydroxy arisanlactone D, 25-hydroxy schindilactone, schindilachone A, and lancifodilactone D are 0.075-1.800, 0.098-0.980; 0.095-0.950, and 0.053-0.530 microg, respectively, and the average recoveries were 98.57%, 96.44%, 97.96%, and 97.27%, respectively.
CONCLUSIONThe four nortriterpenoids were well separated by this method, and it could be used to determine the four nortriterpenoids in Schisandra chinensis extract.
