Determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta by RP-HPLC.
- Author:
Huanyang QI
1
;
Juan CHEN
;
Yanping SHI
Author Information
- Publication Type:Journal Article
- MeSH: Benzaldehydes; analysis; Chromatography, High Pressure Liquid; methods; Resorcinols; analysis; Usnea; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(16):2233-2235
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a RP-HPLC method for determining the contents of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in Usnea diffracta.
METHODA Kromasil-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phases of acetonitrile -1% acetic acid in a gradient manner. The flow rate was set at 1.0 mL x min(-1). The detection wavelength was 280 nm.
RESULTThe correlation coefficients of atranol, lecanorin, ethyl orsellinate, and methyl orsellinate were higher than 0.999. Recoveries were from 102.9% to 95.30%; with RSD from 2.3% to 1.9%.
CONCLUSIONThe method is quick, simple and repeatable for simultaneous determination of atranol, lecanorin, ethyl orsellinate and methyl orsellinate in U. diffracta.
