Ocular pharmacokinetics of puerarin in anesthetic rabbits by microdialysis.
- Author:
Chen YAO
1
;
Xiudi ZHOU
;
Tao QU
;
Dongyu WEI
;
Hongjie MU
;
Rongcai LIANG
;
Aiping WANG
;
Kaoxiang SUN
Author Information
- Publication Type:Journal Article
- MeSH: Anesthesia; Animals; Eye; metabolism; Female; Isoflavones; pharmacokinetics; Male; Microdialysis; methods; Ophthalmic Solutions; Rabbits
- From: China Journal of Chinese Materia Medica 2011;36(16):2236-2239
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the model of microdialysis, and study the ocular pharmacokinetics of puerarin in anesthetic rabbits.
METHODImplanted the probe into anterior chamber of anesthetic rabbit by surgery. After balanced for 2 h, 1% puerarin eye drop (100 microL) was applied into the cul-de-sac with micropipette. Immediately the dialysate was collected at different time and detected by HPLC with the detection wavelength of 249 nm. The mobile phase was methanol and 0.1% citric acid solution (30:70); the flow rate was 1.0 mL x min(-1).
RESULTAfter the administration, puerarin can be absorbed into aqueous humor quickly. The peak concentration of puerarin appeared at about 1 h and then reduced gradually. The peak concentration(C(max)) is (2.52 +/- 0.31) mg x L(-1). The other lower peak was shown at 3.5 h during the eliminate phase. This might be attributed to the inhibition of aqueous humor production by the puerarin and resulted in a high drug concentration. The area under concentration-time curve (AUC(0-t)) is (5.04 +/- 0.21) mg x h x L(-1) and the eliminate half life (t1/2) is (0.38 +/- 0.13) h.
CONCLUSIONThe microdialysis technique can be used to detect the ocular pharmacokinetics of puerarin, and support the valuable pharmacokinetics parameter for the clinical applications of puerarin eye drop.