Puerarin induced apoptosis of pulmonary artery smooth muscle cell by mitochondrial pathway.
- Author:
Xiaodan ZHANG
1
;
Fenghua ZHAO
;
Xiumei ZHANG
;
Jiejing SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 9; metabolism; Cells, Cultured; Isoflavones; pharmacology; Male; Mitochondria; drug effects; Muscle, Smooth, Vascular; cytology; drug effects; Myocytes, Smooth Muscle; drug effects; physiology; Proto-Oncogene Proteins c-bcl-2; analysis; Pulmonary Artery; cytology; drug effects; Rats; Rats, Wistar
- From: China Journal of Chinese Materia Medica 2011;36(16):2255-2258
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the contribution of mitochondrial pathway in the apoptosis induced by puerarin (PUE) in pulmonary artery smooth muscle cells.
METHODCultured rat pulmonary artery smooth muscle cells (PASMC) were intervened by high, middle and low dose of puerarin (1.5 x 10(-3), 1.5 x 10(-4), 1.5 x 10(-5) mol x L(-1)). The change of mitochondrial membrane potential was observed. Western blot detected the expression of apoptosis-related gene Caspase-9, Bax and Bcl-2 protein.
RESULTCompared with the control group, mitochondrial membrane potential significantly decreased in puerarin groups. Puerarin can enhance the expression of Caspase-9 and Bax protein, decrease the expression of Bcl-2 protein. Puerarin also has a concentration-dependent on the induction of PASMC.
CONCLUSIONPuerarin can induce PASMC apoptosis through mitochondrial pathway.
