Simultaneous determination of panaxynol and panaxydol in fibrous root of Panax ginseng by HPLC.
- Author:
Jie LI
1
;
Juan JIANG
;
Yimin ZHENG
;
Linlin WANG
;
Yuqing YANG
;
Yang HU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Diynes; analysis; Drugs, Chinese Herbal; analysis; Fatty Alcohols; analysis; Panax; chemistry; Plant Roots; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(17):2380-2382
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method for simultaneous determination of panaxynol and panaxydol from the fibrous root of Panax ginseng.
METHODThe analysis was performed on Elite C18 column (4.6 mm x 150 mm, 5 microm) with mobile phase gradient of CH3CN-water at a flow rate of 1.0 mL x min(-1). The detection wavelength was 230 nm, and the detection temperature was ambient.
RESULTThe linear range were 0.70-3.50 microg (r = 0.9995) for panaxynol, and 0.64-3.20 microg (r = 0.9999) for panaxydol. The average recoveries were 99.1% (RSD 1.7%) and 99.3% (RSD 1.2%), respectively.
CONCLUSIONThe HPLC method is simple, rapid and reproducible, which can be used for the quality control of the fibrous root of P. ginseng.
