Protective effects and mechanism of hyperin on CoCl2-induced PC12 cells.

Author: Kewu ZENG ¹ ; Xuemei WANG ; Hong FU ; Gengxin LIU
Author Information

1. Research Studio of Integration of Traditional and Western Medicine, First Hospital, Peking University, Beijing 100034, China.
Publication Type:Journal Article
MeSH: Animals; Apoptosis; drug effects; Cell Survival; drug effects; Cells; cytology; drug effects; Cobalt; toxicity; Hypericum; chemistry; PC12 Cells; Plant Extracts; pharmacology; Protective Agents; pharmacology; Quercetin; analogs & derivatives; pharmacology; Rats
From: China Journal of Chinese Materia Medica 2011;36(17):2409-2412
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the neuroprotective effects and mechanism of hyperin on CoCl2-induced hypoxic/ischemic PC12 cells.
METHODCoCl2 was used to treat rat pheochromocytoma cell line (PC12) to investigate the protective effects of different concentrations of hyperin.
RESULTHyperin could significantly inhibit CoCl2-induced cytotoxicity and apoptosis on PC12 cells in a dose-dependent manner, by inhibiting reactive oxygen species (ROS) production, increasing mitochondrial membrane potential (MMP), and inactivating caspase-3 and poly ADP-ribose polymerase (PARP).
CONCLUSIONHyperin could inhibit CoCl2-induced cytotoxicity and apoptosis on PC12 cells, show neuroprotective effects on hypoxic/ischemic neural injuries.