Influence of SGHWJN particles on mediators of inflammation in esophageal tissues of rat with reflux esophagitis.
- Author:
Yongfu QI
1
;
Xuexi WANG
;
Zhong XU
;
Xinwen XU
;
Shang LI
;
Jianxiong ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drugs, Chinese Herbal; administration & dosage; Esophagitis, Peptic; drug therapy; genetics; immunology; Esophagus; drug effects; immunology; Female; Gene Expression; drug effects; Humans; Inflammation Mediators; immunology; Male; Rats; Rats, Sprague-Dawley; Tumor Necrosis Factor-alpha; genetics; immunology
- From: China Journal of Chinese Materia Medica 2011;36(17):2418-2422
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the influence of SGHWJN particles on inflammation and the mediators of inflammation in esophageal tissues of rat with reflux esophagitis.
METHODFifty SD rats were randomly divided into 5 groups, namely, a control group, a sham-operated group, a model group, a SGHWJN particles group and a PPI group. Reflux esophagitis was induced by adopting partial pyloric ligation plus cardiomyotomy. One week later, the rats were orally administered twice daily for 28 days. Pathological changes of esophagus mucous membrane were evaluated by using HE staining and Harry S. Cooper's method in every groups. MDA and SOD contents in esophageal tissues were measured by colorimetric method. Expression of TNF-alpha in esophageal tissues were examined by real-time fluorescent quantitative reverse transcriptase polymerase chain reaction (RT-FQ-PCR) with SYBR Green.
RESULTModel group, esophageal inflammation scores, expression of TNF-alpha in esophageal tissues and MDA contents compared with the normal group and sham operation group were significantly higher (P < 0.05). SOD contents in the esophageal tissues of the model group was significantly lower than that of control group and sham-operated group (P < 0.05). SGHWJN particles group and PPI group of esophageal tissue inflammation scores, expression of TNF-a in esophageal tissues and MDA levels than those in model group decreased significantly (P < 0.05). SOD content was significantly higher than that of model group (P < 0.05), SGHWJN particles group and PPI group showed no statistically significant difference between the above-mentioned indicators. The above-mentioned indicators showed no statistically significant difference between the normal group and sham-operated group. MDA content and expression of TNF-alpha in esophageal tissue was positively correlated with inflammatory scores of model group (r = 0.813). Model group esophageal tissue SOD content and inflammation scores were negatively correlated (r = -0.847). Esophageal tissue SOD levels were negatively correlated with MDA levels (r = -0.863).
CONCLUSIONSGHWJN particles can effectively inhibit inflammation in rat with reflux esophagitis through regulating TNF-alpha, SOD and MDA.
