Relationship between hepatitis B virus YMDD mutation and serum viral DNA loadings.
- Author:
Ling-He KONG
1
;
Su-Xiang GAO
;
Ya-Ping GUI
;
Wen-Hong LIU
Author Information
- Publication Type:Journal Article
- MeSH: Antiviral Agents; pharmacology; DNA, Viral; blood; Drug Resistance, Viral; genetics; Female; Hepatitis B; blood; Hepatitis B virus; drug effects; genetics; physiology; Humans; Lamivudine; pharmacology; Male; Middle Aged; Mutation; Viral Load; genetics
- From: Journal of Southern Medical University 2007;27(8):1262-1263
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between lamivudine-resistant mutants of hepatitis B virus (HBV) and serum HBV DNA loading before antiviral therapy.
METHODSThis study involved 106 patients with hepatitis B receiving lamivudine treatment for an average of 32 months (rang 12-48 months). Serum HBV DNA loadings were measured with PCR before and every 4 to 6 months during lamivudine therapy. HBV YMDD mutants were detected using mismatched PCR-restriction fragment length polymorphism (PCR-RFLP) during lamivudine treatment.
RESULTSHBV DNA loading was significantly higher in patients infected with HBV YMDD mutants during lamivudine therapy than those infected with HBV without YMDD mutation.
CONCLUSIONHigh viral loading in hepatitis B patients before treatment is associated with high likeliness of HBV YMDD mutation during lamivudine treatment. HBV DNA loading may be indicative for the occurrence of YMDD mutation during lamivudine therapy.
