Efficient and rapid non-test tube cloning of Jatropha curcas.
- Author:
Zhao-Yu WANG
1
;
Jing-Ming LIN
;
Zeng-Fu XU
Author Information
- Publication Type:Journal Article
- MeSH: Cloning, Organism; methods; Jatropha; drug effects; genetics; growth & development; Plant Growth Regulators; pharmacology; Plant Roots; drug effects; genetics; growth & development; Temperature; Time Factors
- From: Journal of Southern Medical University 2007;27(8):1285-1286
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a new technique for efficient and rapid non-test tube cloning of the medicinal and energy- producing plant Jatropha curcas.
METHODSUsing the mini-stem fragment (2-3 cm) of Jatropha curcas with merely one axillary bud as the explant, the effect of an auxin IBA concentration on the plantlet regeneration was studied.
RESULTS AND CONCLUSIONWhen treated with 1 mg/LIBA for 1h, the explants showed the most rapid propagation. The mini-stem fragments high root regeneration ratio (96.7%), short root regeneration period (18.2-/+2.0 d), large number of new roots per explant (6.3-/+1.8), and long total root length (6.8-/+3.5 cm), demonstrating that this technique can be a simple and efficient method for rapid non-test tube cloning of Jatropha curcas of potential industrial value.
