Effect of small interfering RNA-induced LKB1 gene silencing on the biological behavior of lung carcinoma cells.
- Author:
Xuan LIANG
1
;
Ke-jun NAN
;
Qin-zhi XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; genetics; Blotting, Western; Cell Line, Tumor; Cell Proliferation; Flow Cytometry; Gene Silencing; Humans; Lung Neoplasms; genetics; pathology; Protein-Serine-Threonine Kinases; deficiency; genetics; RNA, Small Interfering; genetics
- From: Journal of Southern Medical University 2007;27(9):1303-1306
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of the small interfering RNA (siRNA)-mediated LKB1 gene silencing on the biological behavior of lung carcinoma cells.
METHODSRNA interference technique was used to silence LKB1 gene in lung carcinoma cells, and the expression of LKB1 protein were subsequently detected by Western blotting. The cell proliferation was then assessed by observing the growth curve and clone formation of the cells, and cell cycle and apoptosis were assayed by flow cytometry.
RESULTSLKB1 siRNA resulted in remarkable suppression of LKB1 expression in the lung carcinoma cells. LKB1 gene silencing resulted in accelerated cell proliferation, but cell apoptosis underwent no significant changes.
CONCLUSIONDown-regulation of LKB1 gene expression can stimulate malignant biological behavior of lung carcinoma cells.
