Role of AT2 receptors on angiotensin II-induced tumor necrosis factor alpha and interleukin 1 beta secretion in adult rat cardiac fibroblasts.
- Author:
Xiao-ying JIANG
1
;
Guang-dao GAO
;
Juan ZHOU
;
Rong GUO
;
Yuan-xi LI
Author Information
- Publication Type:Journal Article
- MeSH: Angiotensin II; pharmacology; Animals; Fibroblasts; drug effects; metabolism; secretion; Gene Expression Regulation; drug effects; Interleukin-1beta; secretion; Male; Myocardium; cytology; Rats; Rats, Sprague-Dawley; Receptor, Angiotensin, Type 2; metabolism; Tumor Necrosis Factor-alpha; secretion
- From: Journal of Southern Medical University 2007;27(9):1307-1309
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of AT2 receptors in the secretion of tumor necrosis factor alpha (alpha-TNF) and interleukin 1 beta (IL1 beta) in adult rat cardiac fibroblasts.
METHODSAdult rat cardiac fibroblasts in in vitro culture were divided into control, Ang II, AngII + Losartan, and AngII + PD123319 groups with corresponding treatments. Radioimmunoassay was used to determine alpha-TNF and IL1 beta levels in the supernatant of the treated cardiac fibroblasts.
RESULTSAng II treatment resulted in significantly increased alpha-TNF and IL1 beta levels. Compared with AngII group, IL1 beta level was decreased by 69.1% and 78.7% and alpha-TNF by 58.7% and 65.9% after blocking AT1 and AT2 receptors, respectively.
CONCLUSIONAT2 receptors are involved in alpha-TNF and IL1 beta secretions in cardiac fibroblasts.
