Expression of DNA damage checkpoint mediator 1 and p53-binding protein 1 in human esophageal cancer cell lines TE-1, TE-13, and Eca109.
- Author:
Zhi-kun LIU
1
;
Shu-chai ZHU
;
Yu-xiang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blotting, Western; Cell Line, Tumor; DNA Damage; genetics; Esophageal Neoplasms; genetics; metabolism; pathology; Flow Cytometry; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Intracellular Signaling Peptides and Proteins; genetics; metabolism; Nuclear Proteins; genetics; metabolism; RNA, Messenger; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Trans-Activators; genetics; metabolism; Tumor Suppressor p53-Binding Protein 1
- From: Journal of Southern Medical University 2007;27(9):1314-1317
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the expression of DNA damage checkpoint mediator 1 (MDC1) and p53-binding protein 1 (53BP1) at both mRNA and protein levels and their significance in different human esophageal cancer cell lines.
METHODSIn 3 human esophageal carcinoma cell lines, TE-1, TE-13 and Eca109 cells, the expressions of MDC1 and 53BP1 mRNA were detected with RT-PCR, and MDC1 and 53BP1 protein expressions were measured with immunohistochemistry, indirect immunofluorescence and Western blotting, respectively.
RESULTS AND CONCLUSIONSMDC1 and 53BP1 expressions were observed for the first time in human esophageal carcinoma cell lines TE-1,TE-13 and Eca109 cells, at both the mRNA and protein levels. The expressions of MDC1 and 53BP1 proteins may be implicated in the radiosensitivity of human esophageal carcinoma.
