Expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs.
- Author:
Qiong LIU
1
;
Jie WU
;
Xin-mei WANG
;
Jun-wen ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Female; Gene Expression Regulation; Guinea Pigs; Immunohistochemistry; Male; Myopia; metabolism; pathology; RNA, Messenger; genetics; metabolism; Receptors, Muscarinic; genetics; metabolism; Sclera; cytology; metabolism
- From: Journal of Southern Medical University 2007;27(9):1327-1330
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of 5 muscarinic receptor subtypes in the scleral tissue of immature guinea pigs.
METHODSThe scleral tissue was collected from six 2-week-old pigmented guinea pigs to determine mRNA expressions of the muscarinic receptors with RT-PCR. Immunofluorescence was used to observe the protein expressions of the 5 muscarinic receptor subtypes.
RESULTSThe mRNAs of the 5 muscarinic receptor subtypes were all detected in the scleral tissue, and the mRNA expression was the highest for M1 subtype. Green fluorescence of M1 to M5 subtypes surrounding the fibroblast nuclei were found in the scleral matrix using laser confocal microscopy.
CONCLUSIONFive muscarinic receptor subtypes are present in the scleral tissues of immature guinea pigs, indicating the involvement of the muscarinic receptors in eye growth regulation.