Dynamic analysis of HBV cccDNA in HepG2 cells infected with Ad-1.2 HBV.
- Author:
Wei-fang LIANG
1
;
Zhi-hua LIU
;
Jie YANG
;
Kang-xian LUO
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Calibration; Cell Line, Tumor; DNA, Complementary; genetics; metabolism; DNA, Viral; genetics; metabolism; Hepatitis B Surface Antigens; metabolism; Hepatitis B e Antigens; metabolism; Hepatitis B virus; genetics; immunology; metabolism; physiology; Humans; Polymerase Chain Reaction; Time Factors; Transfection; Virus Replication
- From: Journal of Southern Medical University 2007;27(9):1374-1375
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEo study the replication of hepatitis B virus (HBV) in HepG2 cells infected with Ad-1.2 HBV.
METHODSHepG2 cells were transfected with adenovirus containing 1.2 copies of HBV DNA. The expression of HBV antigens were detected in the culture medium by means of enzyme-linked immunosorbent assay (ELISA), and the covalently closed circular DNA (cccDNA) in the cells was extracted with plasmid extraction kit and detected by real-time PCR with selective primer after treatment with mung bean nuclease.
RESULTSHBsAg, HBeAg and HBV cccDNA were all detected in HepG2 cells after tranfection with Ad-1.2 HBV. HBV cccDNA was detected 1 day after the infection, reaching the peak level 4 days after infection.
CONCLUSIONAd-1.2 HBV-infected cells can serve as the model for screening and evaluation of antiviral agents.
