Effects of specific small interfering RNA on Smoothened expression and LoVo cell proliferation and apoptosis.
- Author:
Da-jian ZHU
1
;
Chi-hua FANG
;
Zhen-xiang RONG
;
Sheng-jun LIU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; genetics; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; genetics; Humans; RNA, Small Interfering; genetics; Receptors, G-Protein-Coupled; deficiency; genetics; Smoothened Receptor; Time Factors; Transfection
- From: Journal of Southern Medical University 2007;27(9):1414-1417
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of specific small interfering RNA (siRNA) on Smoothened (Smo) gene expression and the proliferation and apoptosis of colorectal cancer LoVo cells.
METHODSThree different siRNAs (siRNA-1, siRNA-2, and siRNA-3, respectively) were transfected into LoVo cells via cationic liposome, and the changes of Smo mRNA level were determined using semi-quantitative RT-PCR 48 h after transfection. Flow cytometry and MTT assay were performed to assess the effect of the siRNAs on the proliferation and apoptosis of LoVo cells.
RESULTSForty-eight hours after Smo siRNA-1 transfection, Smo mRNA expression in LoVo cells decreased by about 63.56%, a reduction significantly greater than that in cells transfected with the other two siRNAs. The cell proliferation decreased significantly after Smo siRNA-1 transfection in comparison with the control cells, and 48 h after transfection, significantly higher apoptosis rate was observed in Smo siRNA-1-transfected cells than in the control cells.
CONCLUSIONSpecific siRNA can significantly decrease Smo mRNA expression and inhibit the proliferation while inducing apoptosis of LoVo cells.
