Expression of HSF1 and XAF1 in gastro-intestinal cancer.
- Author:
Ting LI
1
;
Cun-long CHEN
;
Ji-de WANG
;
Sheng-da CUI
;
Dan-yu CUI
;
Wen GUO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Base Sequence; Cell Line, Tumor; DNA-Binding Proteins; genetics; Gastrointestinal Neoplasms; genetics; metabolism; pathology; Gene Expression Regulation, Neoplastic; Heat Shock Transcription Factors; Humans; Intracellular Signaling Peptides and Proteins; Neoplasm Proteins; genetics; Oxidative Stress; genetics; Temperature; Transcription Factors; genetics
- From: Journal of Southern Medical University 2007;27(9):1447-1450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEX-linked inhibitor of apoptosis protein (XIAP) To gastrointestinal (GI) investigate the expression of XAF1 and heat-shock transcription factors 1 èHSF1éand their relationship in human gastrointestinal cancers.
METHODSImmunoblotting was used to analyze the expression of HSF1 and XAF1 in either gastric or colon cancer tissue and GI cancer cell line. Transient expression of the HSF1-containing vector in GI cell lines and RNA interference were used to up/down-regulae the expression of the HSF1, and the subsequent expression of XAF1 was measured.
RESULTSThe expression of HSF1 was higher in GI cancers than in normal tissues. The expression of XAF1 and HSF1 was negatively correlated in GI cancer cell lines. Stress stimuli up-regulated the expression of HSF1 while the alteration of XAF1 expression was negatively correlated with HSF1 expression.
CONCLUSIONThe high expression of HSF1 in GI cancers is associated with suppressed expression of XAF1, which can be one of the mechanisms for low-expression of XAF1 and insufficient apoptosis in GI cancers.
