Inhibitory effect of polysaccharides on the six-alpha-helix bundle formation of HIV gp41 protein.
- Author:
Xiao-Yun WEN
1
;
Shao-Yu WU
;
Wei XU
;
Li LV
;
Shu-Wen LIU
;
Jin-Jun RAO
;
Jia-Jie ZHANG
;
Guang-Fa WANG
;
Shan-He WAN
;
Shu-Guang WU
Author Information
- Publication Type:Journal Article
- MeSH: HIV Envelope Protein gp41; chemistry; Kinetics; Oryza; chemistry; Polysaccharides; pharmacology; Protein Structure, Secondary; drug effects; Reishi; chemistry; Streptomyces; chemistry
- From: Journal of Southern Medical University 2007;27(10):1498-1500
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare the in vitro inhibitory effect of expolysaccharides from Streptomyces, polysaccharides of Ganoderma lucidum and rice bran on six-alpha-helix bundle formation of HIV gp41 protein.
METHODSThe amount of six-alpha-helix bundle formed in the presence of N36 and C34 was tested by ELISA in response to treatments with different doses of polysaccharides.
RESULTSExpolysaccharides from Streptomyces potentially inhibited six-alpha-helix bundle formation with the effective concentration (IC(50)) of 145.48-/+7.25 mg /L. Polysaccharides of Ganoderma lucidum and rice bran showed no effect on the six-alpha-helix bundle formation.
CONCLUSIONExpolysaccharides from Streptomyces can inhibit the six-alpha-helix bundle formation of HIV gp41, whereas polysaccharides of Ganoderma lucidum and rice bran do not exhibit such activity.
