Green fluorescent protein gene transfection mediated by recombinant adenovirus vector to rat bone marrow mesenchymal stem cells.
- Author:
Xia-Fei FU
1
;
Yuan-Li HE
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; metabolism; Animals; Bone Marrow Cells; cytology; metabolism; virology; Cell Proliferation; Cells, Cultured; Female; Genetic Vectors; genetics; metabolism; Green Fluorescent Proteins; genetics; metabolism; Male; Mesenchymal Stromal Cells; cytology; metabolism; virology; Mice; Rats; Rats, Wistar; Transduction, Genetic; methods
- From: Journal of Southern Medical University 2007;27(10):1517-1520
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the efficient transfection of green fluorescent protein gene (GFP) mediated by recombinant adenovirus vector(Ad-GFP) to rat bone marrow mesenchymal stem cells (MSCs) in vitro.
METHODSWistar rat bone marrow-derived MSCs were separated and purified in vitro by Percoll density gradient centrifugation combined with adherent cell culture followed by identification with flow cytometry. MSCs infected by Ad-GFP were observed and the transfection efficiency was assessed by fluorescence microscope. The proliferative ability of these cells was tested by CCK-8.
RESULTSThe transfection efficiency was as high as 90.0%. Expression of GFP gene of infected MSCs was stable for 1 month after infection. There was no statistically difference in proliferative ability between the infected MSCs and non-infected ones (P>0.05).
CONCLUSIONThe infected MSCs with Ad-GFP expressed GFP with high efficiency and retain the ability of proliferation as non-infected MSCs. Transgection with Ad-GFP is a highly effective method for labeling MSCs.
