Effects of exogenous human chorionic gonadotropin on nude mice bearing endometrial carcinoma.
- Author:
Jun-Sheng ZHENG
1
;
Min HUANG
;
Chen-Li LIN
;
You-Ke XIE
;
Ming-Hua WANG
;
Xue-Yun ZHONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line, Tumor; Chorionic Gonadotropin; therapeutic use; Disease Models, Animal; Endometrial Neoplasms; drug therapy; genetics; metabolism; Female; Gene Expression; Humans; Ki-67 Antigen; genetics; metabolism; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Random Allocation; Xenograft Model Antitumor Assays
- From: Journal of Southern Medical University 2007;27(10):1531-1534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of exogenous human chorionic gonadotropin (hCG) on nude mice bearing transplanted endometrial carcinoma.
METHODSHuman endometrial carcinoma xenograft was transplanted in nude mice, and the effects of hCG injection on the tumor growth was evaluated according to tumorigenesis and xenograft weights. The expression of Ki-67 in the tumor was determined by immunohistochemistry, and HE staining was performed for morphological observation and measurement of the necrosis area in the tumor. The effect of hCG on fibrosis in the tumor was evaluated with Masson staining.
RESULTSCompared to normal saline-treated tumor-bearing mice, the mice with hCG treatment showed increased tumor weight. HE staining for tumors in HCG-treatment group visualized tumor cell arrangement in glandular structure with smaller necrosis area, and Masson staining identified thick and compact collagen fibers as compared with the thin and loosely arranged fibers in saline-treated group. No significant difference was found in the Ki-67 expression in the tumors between the two groups.
CONCLUSIONExogenous hCG can promote the differentiation of the endometrial carcinoma cells in vivo.
