Time-resolved fluoroimmunoassay for detecting hepatitis B virus surface antigen.
- Author:
De-Qiu LIN
1
;
Jian SHEN
Author Information
1. College of Life Sciences, South China Normal University, Guangzhou 510631, China.
- Publication Type:Journal Article
- MeSH:
Fluoroimmunoassay;
methods;
Hepatitis B;
blood;
Hepatitis B Surface Antigens;
blood;
Humans;
Sensitivity and Specificity
- From:
Journal of Southern Medical University
2007;27(10):1575-1576
- CountryChina
- Language:Chinese
-
Abstract:
A convenient, highly sensitive and highly specific method using time-resolved fluoro immunoassay (TRFIA) for quantitative detection of hepatitis B virus is described. Using EU-DTTA as the tracer molecule, the assay showed a detection range of the standard curves of 0.2-300 ng/ml for HbsAg with a sensitivity of 0.05 ng/ml. The within-run coefficient of variations for standard samples were less than 10%, and the correlation coefficient between radioimmunoassay and TRFIA assay results reached 95% for the same sample, demonstrating the advantages of TRFIA for its wide detection range, high sensitivity and simple operation and its great potentials for clinical use.
