Synergistic effects of acitretin and narrow-band ultraviolet-B in inducing retinoic acid receptor gamma mRNA expression in normal human keratinocytes.
- Author:
Su-ju LUO
1
;
Zhen-hui PENG
;
Yan ZHENG
;
Hao-xiang XU
;
Shao-na ZHOU
;
Xiao-li LI
;
Guo-rong WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acitretin; pharmacology; Cells, Cultured; Humans; Keratinocytes; drug effects; radiation effects; RNA, Messenger; metabolism; Receptors, Retinoic Acid; metabolism; Ultraviolet Rays
- From: Journal of Southern Medical University 2007;27(11):1631-1634
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes in cell proliferation and retinoic acid receptor gamma (RARgamma) mRNA expression in normal human keratinocytes after acitretin treatment and/or narrow-band ultraviolet-B irradiation.
METHODSNormal human keratinocytes were exposed to irradiation with 100 mJ/cm square NB-UVB and/or subsequent 12-hour incubation with 1x10(-6) mol/L acitretin, and the expression of RARgamma mRNA in the cells was examined using RT-PCR and real-time quantitative RT-PCR.
RESULTSA 0.9- and a 2.3-fold increase in RARgamma mRNA expression was induced in the cells by exposure to 100 mJ/cm square NB-UVB and 10(-6) mol/L acitretin, respectively, and the expression was synergistically enhanced by 2.8-fold after their combined treatment.
CONCLUSIONUpregulated expression of RARgamma mRNA can be associated with keratinocyte growth inhibition after treatment with acitretin and NB-UVB irradiation.
